The program teaches about ligand binding and its applications
in pharmacology at elementary and advanced levels.
It is up to the tutor to set objectives for particular groups of students
and to determine which parts of the program will be beneficial.
It can be used in conjunction with lectures or as a replacement,
for initial or remedial teaching of undergraduates or for postgraduates
who need to become familiar with the basis of this technique.
The program deals with:
- The characteristics of ligands and binding sites and the bonds
which form between them
- The parameters measured by ligand binding assays (dissociation
constant, number of sites)
- The methodology of ligand binding assays:
- The derivation of binding constants from saturation assays including
Saturation plot, Scatchard plots, Hill plots, double reciprocal
plots and the basis of these data treatment methods:
- One, two and three specific binding site models and the influence
of non-specific binding
- Competitive binding assays including use and data processing
- The application of ligand binding in pharmacology
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