9b. Appendix B - Transcript of Open Ended Questionnaire Responses B2. Experiments on Isolated Guinea Pig Ileum B2.1 Bath STUDENT ID WHAT DO YOU THINK YOU WERE MEANT TO LEARN FROM THE PACKAGE? FROM THE SECTIONS YOU STUDIED, WHAT, IF ANYTHING, DID YOU FIND EASY TO GRASP? FROM THE SECTIONS YOU STUDIED, WHAT, IF ANYTHING, DID YOU FIND DIFFICULT TO GRASP? ANY FURTHER COMMENTS OR OBSERVATIONS 20191 - Experimental set up explanations. Not done enough of the package. 1 hr 30 min. 20281 How agonists work. - - About 2 ½ hours. 20481 The affect of drugs on the guinea pig ileum and it's structure, differences in mode of action of agonist/antagonists. - - 1 ¾ hours. 20591 - Not really anything specifically. Chemical aspects. Quite a lot of the biological aspects due to my lack of biological background. 2 hours. 20691 The effects of different drugs on the contraction of longitudinal muscle of the ileum. Just about everything. Nothing About 1 ½ - 2 hours. 20781 Different agonists have differing responses on identical samples of tissue. Application of Dose Response curves. Basic premise of Receptor theory, i.e. What an agonist is, what an antagonist is, partial agonists etc. Idea of plotting graphs to compare different drugs/concn - Currently approximately 1 ½ - 2 hours; package seems pedantic at times, when checking answers for concn of ions and isotonic solutions and swapped identical ones over and mine was "wrong" despite the right figures. 20891 Dose response curves - what they are, how to construct them - and how the agonists vary in their affect. - - 2 ½ hours. 20991 What agonists are and how they affect their receptors. How different agonists affect guinea pig ileum and how this effect varies with concentration of agonist. All of it. - 2 hours. 21091 About agonists/antagonists and dosage response curves. - - 1 ½ hours. 21181 Showing the effects of various stimulants on a piece of guinea pig ileum. The principle of inc concentrations will only cause response to increase up to a certain level. - 1 hr. 21291 The effects of diff concs of diff substance on the tissue and how different mechanism of action effect care needed e.g., Ka and acelytcholine. The effect of the agonists on the material and how it changed and why. Nothing About 2 ½ hours so far. 21393 About the action of drugs on isolated Guinea Pig Ileum. It was all fairly easy to understand. - 2 hours. 21481 The effect of agonists on the pig ileum. - - Constantly changing agonist concentrations was tedious. A week gap between doing package and test. 21581 To increase my knowledge of agonists and their effects. - - I have spent about 2 hrs on the PCCAL package. 21693 What agonists are and the way different agonists have different effects on receptors, also the way agonists work can be altered. The structure of the ileum: the construction of curves. - 2 ½ hours. 21791 How to set-up and conduct the experiment, the aims of the experiment. The experimental set-up, the diagrams and illustrations made it easier. Initially, what I was suppose to do experiment-wise with the agonist ie. How and when I was suppose to alter the concentrations. 2 ½ hrs. 21893 The action of agonists on the contraction of the longitudinal muscle of the guinea pig ileum. Structure of the ileum, difference in magnitude of contractions according to different agonists. Identification of the receptor sites, e.g., musaninic or nicotinic etc. 2 hours. 21991 About agonists in general and especially those acting on smooth muscle. Dose response curves and EC50's, apparatus used in experiment. All of them were fairly easy to understand. - 2 ½ hours. The package was well laid out and each new "subject" to learn was introduced at the right 'pace'. The experiment was easy to understand, and being able to look back through the theory pages helped too. 22081 The effect of increasing concentration of the agonist and antagonist the contraction of the ileum, and also the difference between the two. It was generally easily to follow. - The package took about an hour and a half. 22191 How the experiment works, and what happens when different drugs are put in contact with the ileum. Understanding of what happened in the experiment. Straight facts that were not known before, a lot were new and hard to take in all at once. 2 ¼ hours - the package was more interesting and easy to follow than the frog one and seemed more relevant. 22291 What an agonist and antagonist are, and how sensitive receptors are to these substances. The concept that low concentrations of drugs can produce responses. The experimental set up. 2 ¼ hours. The package was quite useful because we had done no previous work on the subject, so it taught a lot of new facts. It wasn't too boring either. 22381 The action of drugs on receptor sites. The use of agonists and effect of concentration of agonists. The experimental set up, and the construction and comparison of dose response curves were covered very well. The topic which dealt with receptors. Time spent working with the package was about 2 ½ hours. 22491 Example of how various agonists cause responses and how to record the data. Most of the ideas and concepts. How to produce log concentration against % max response curve was not entirely clear. Two hours. 22592 About agonists/antagonists, and constructing dose response curves. - - About 2 hours, though much of this was just copying data from the screen. 22691 What agonists are, how the experiment worked, the equipment used in this sort of experiment. How the experiment worked was easy to grasp. Why the agonists produced the response they did was something I found difficult to grasp. About an 1 ½ - 2 hours. 22781 That different [] agonists can cause different contraction sites. Occupancy of receptors. Naming various parts on a diagram. ~ 1 ½ hours. 22891 About dose response curves. The set-up of experiment. The anatomy of the ileum. 1 ½ - 2 hrs. 22991 The procedure for conducting the experiment and the data you might expect to collect. - - 2 hours. 23091 How to construct dose-response curves, how to set up tissue to be examined, the effects of different agonists on guinea-pig ileum. How to set up ileum, the meaning of agonist. - 2 hours. 23191 We were meant to learn about the effects of different drugs on the action of the smooth muscle of the guinea-pig ileum. Structure of the ileum, using the package to record the effects of the drugs, the apparatus of the experiment and how it worked. - Approx. 1 ½ hours. 23292 About agonists. - - 2 - 2 ½ hours. 23391 The variance in how different agonists work, on guinea pig ileum. How the curves were produced. - Approx. 2 hrs. I was very helpful and my understanding improved the further into the package I got. Although it will take a few more hours to be completely confident. 23481 Basic understanding of Agonists and Antagonists. The meaning of the terms. - About 1 hour 40 minutes. 23591 - - - 2.5 hrs. 23693 Effects of agonists on tissues with respect to contraction. Agonists - theory. Agonists - constructing dose response curves. 3 hours:- I request that information for e.g., type of curve especially regarding construction of the curves should be detailed. 23791 How to construct dose response curves and the effects of agonists on tissue ie ileum. When changing the concn of agonist, recording the numbers given and predicting the outcome. - About 1 hour. 23881 - - - 2:30 hrs. 23991 Experimental method of measuring muscular response to different agonists. The principal of the experiment and how it worked. - 2 hours. It was a clear package that was well explained and illustrated. 24091 The action of agonists on a guinea pig ileum and the difference between various agonists. The experimental set up. The structure of the ileum and the sympathetic and parasympathetic receptors. 2 ½ hrs. Changing the concentration of agonist for each simulation was laborious, could it be done in preset stages, those practised in the lab, as an alternative. At present it is four clicks… This could be two … For example x All displayed on screen. Simply click to change. 24193 Response curves, agonist and antagonist action of tissues. Diagrammatic stuff. Explanation of how an experiment work. 3 hours, I like the packages, I can learn more thing. 24291 The action of agonists and how response varies with concentrations and types of agonist. - How to use the program to construct data response curves. Two hours. 24391 Construction of dose response curves. Effect of agonists and antagonists on guinea pig ileum. - - - 24491 How different agonists and different concentrations affect the amount of contraction of the ileum. Everything. No 3 hours. 24591 How agonists affect the smooth muscle in the body from the experiment port. Most things. Remembering things like the concentration in the Tyrodis soln 3 hour practical 24691 How experiment work and the effects of different drugs on the guinea pig ileum. All sections easy to grasp. None About 3 hours. 24891 How agonists and antagonists work. - How experiment was set up. Approximately 3 hours on package itself, and some more time reading up. 24991 The effect of agonists on guinea pig ileum. Experimental set up. - About two hours. The agonist theory was clearly set out but a few more instructions for the final part may have been useful rather than making it up as we went along. 25081 To learn about receptors and agonist relationships and effects of 2 combining. The sections were all relatively straightforward and reasonably easy to grasp. - 2 hrs was spent on the package Õ quite useful and straightforward. 25291 How concn of agonists effect muscle contraction. How the experiment is set up. How the expt worked. What conc to initially start with. Approximately 2 hours. 25381 - The structure of the gut due to the help of the diagrammatic representation. Interpreting the dose response curves. ~ 3 hours. 25491 - The package was very user-friendly and so was easy to work through. No. 3 hours. 25581 What an antagonist is and what an agonist is, and relate this to work done on computers and in lectures. The theory work was easy to grasp. Constructing dose response curves. About 2 hours. 25681 - Most of it. None. 2 hours. 25781 What the definitions were for agonist and antagonists, etc. It was easy to grasp the experimental procedure. Nothing. 2 hours. 25891 Background of the experimental set-up and effects of agonist and antagonists. The definitions questions in the Theory section, e.g., What is an Agonist? The diagrams of the ileum etc. which we had to label were difficult. Two hours. 25981 Diff. bet agonists/antagonists. The relationship of agonist/conc. - 2 hrs. 26081 Effect of agonists on the ileum over a concn range. Agonist theory. - - 26191 The experimental aspects and the theory of Drug Response. The diagram of the ileum wall and the labels. - 3 hrs. 26281 How to construct dose response curves. - - 2 hrs. 26383 How the guinea pig ileum muscles respond to different concentration of drugs. Agonists - Theory. Agonists - Constructing the Dose Response Curves. Experimental set-up. Approximately 2 hours. The package is very helpful but with more time I think people can grasp what they require to. 26491 The basic structure of the ileum, how certain drugs effect it in their various concentrations and how to plot dose response curves. Most of the package was easy to follow and easy to understand. Plotting the dose response curves and understanding them was easy. Nothing. I spent about an hour and a half on the package. 26591 The affect of agonists on muscle - which of the agonists were most potent and which ones acted ont he muscle in the gut. How to actually do the experiment and the affects of agonists. (N/A) About one hour. 26691 How agonists affect the ileum. - - 2 hours. 26791 The structure of the guinea pig ileum. How to set up an experiment to determine the relationship between agonists and response of the guinea pig ileum, and the relationship between agonist and response. The experimental set up. - About 1 ½ hours. 26881 How to use the topics/packages and learn about them. Receptors. Dose-response curves. About 2 hours, my confidence has risen slightly only though as the actual topic is relatively difficult, i.e., after only 2 hrs. you don't feel like you know it all! 27091 The effects of different agonists on the ileum. Construction of the graphs. The concentration calculation. 2 hours. 27181 About guinea pigs and drug responses. How the system worked. What the idea of the experiment was ie. To find a peak value for each drug. 2 hours, the package was useful as it meant experiments could be repeated simply and easily. 27293 I learned the experimental set up for the agonists on the Guinea Pig ileum. Agonists - theory. Agonists - comparing dose response curves.   27391 Relative potency of agonists. - - - 27581 The effect of different agonists on muscles. Understanding of theories, experimental technique. Experimental set up. 2 hours. Tedious when working through the actual experiment. 27691 About guinea pigs. How the expts. worked. - Approx. 2 hrs. 25191 No Paper - - - 24793 Yes. The structure of ileum. The experimental set up. Receptors and their sites of actions. I have spent about 2 hours, studying the experimental - set up only.